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  1. 首頁 > 生化試劑 > 多肽 > 肽表位 > FLAG Peptide TFA salt

FLAG Peptide TFA salt

    
98%

DYKDDDDK Peptide TFA salt

源葉(MedMol)
S81180 一鍵復(fù)制產(chǎn)品信息
98849-88-8(free base)
C41H60N10O20
1012.97
貨號(hào) 規(guī)格 價(jià)格 上海 北京 武漢 南京 購買數(shù)量
S81180-5mg 98% ￥544.00 7 - - -
S81180-10mg 98% ￥829.60 7 - - -
S81180-25mg 98% ￥1224.00 8 - - -
S81180-100mg 98% ￥3264.00 7 - - -
產(chǎn)品介紹 參考文獻(xiàn) 質(zhì)檢證書(COA) 摩爾濃度計(jì)算器 相關(guān)產(chǎn)品

產(chǎn)品介紹

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

產(chǎn)品描述：

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.
靶點(diǎn)： Peptides;Others
體外研究： Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions。
參考文獻(xiàn)： 1. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.
2. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.
溶解性： Soluble  in  H2O
保存條件： -20℃
配置溶液濃度參考：
1mg 5mg 10mg
1 mM 0.987 ml 4.936 ml 9.872 ml
5 mM 0.197 ml 0.987 ml 1.974 ml
10 mM 0.099 ml 0.494 ml 0.987 ml
50 mM 0.02 ml 0.099 ml 0.197 ml
注意： 部分產(chǎn)品我司僅能提供部分信息，我司不保證所提供信息的權(quán)威性，僅供客戶參考交流研究之用。

參考文獻(xiàn)

質(zhì)檢證書(COA)

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請(qǐng)輸入貨號(hào)和一個(gè)與之匹配的批號(hào)。
例如:
批號(hào)：JS298415 貨號(hào)：S20001-25g
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摩爾濃度計(jì)算器

質(zhì)量 (mg) = 濃度 (mM) x 體積 (mL) x 分子摩爾量 (g/mol)

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