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脂多糖

    
BR,來源于大腸桿菌 055:B5

Lipopolysaccharides(LPS)

源葉
S11060 一鍵復制產(chǎn)品信息
LPS 內(nèi)毒素 細菌內(nèi)毒素
貨號 規(guī)格 價格 上海 北京 武漢 南京 購買數(shù)量
S11060-10mg BR,來源于大腸桿菌 055:B5 ¥450.00 >10 >10 - -
S11060-100mg BR,來源于大腸桿菌 055:B5 ¥3600.00 >10 3 - -
產(chǎn)品介紹 參考文獻(135篇) 質(zhì)檢證書(COA) 摩爾濃度計算器 相關(guān)產(chǎn)品

產(chǎn)品介紹

 脂多糖,英文名稱Lipopolysaccharides,簡稱LPS,是革蘭氏陰性細菌細胞壁中的一種特有成分,位于細胞壁的最外層并暴露于非莢膜細菌的細胞表面,有利于維持細胞外膜的完整性,保護細菌免受膽汁鹽和脂類抗生素的破壞。結(jié)構(gòu)上,脂多糖由類脂A、核心多糖和O-多糖側(cè)鏈組成。其中類脂質(zhì)A是構(gòu)成細菌內(nèi)毒素的主要成分,決定其毒性強弱;而O-多糖側(cè)鏈在不同細菌間是高度變化的,特異性決定細菌的血清型。

LPS可以引起免疫刺激的級聯(lián)反應和機體的毒性病理生理活動,包括釋放內(nèi)毒素引起感染性休克從而導致末梢血管虛脫。臨床常通過檢測LPS的存在來診斷腦膜炎。正是LPS與機體免疫機能的密切關(guān)系,生命科學研究常常提取LPS進行相關(guān)的研究,如闡明LPS的結(jié)構(gòu),代謝,免疫學,生理學,毒性,生物合成途徑;誘導生長促進因子如白介素的合成與分泌;誘導疾病研究的動物模型如炎癥反應,急性肺損傷。將LPS分別與FITC、TRITC或2,4,6-trinitrobenzene sulfonic acid (TNBSA)反應即可得到FITC、TRITC或TNP標記的LPS,主要用于非胸腺依賴性B細胞對細菌LPS的免疫應答研究。。

LPS可通過TCA、酚、酚-氯仿-石油醚等方法制備,其中最后一種方法為粗提取法。TCA和酚提取得到的LPS在結(jié)構(gòu)上、電泳圖譜和內(nèi)毒素水平均較相似,不同之處在于含有的核酸和蛋白質(zhì)殘留量不同,TCA法得到的LPS含有約2%的RNA和10%的變性蛋白。酚法提取的LPS含有達60%的RNA和少于1%的蛋白。用凝膠過濾層析可除去酚提取LPS中的殘留蛋白,但是仍會留下約10-20%的核酸。進一步用離子交換層析法純化可得到<1%蛋白和<1%RNA的LPS。

本品來源于血清型大腸桿菌O55:B5,用苯法提取而得。其內(nèi)毒素水平不少于500,000 EU (endotoxin units)/mg,經(jīng)分析,1ng相當于0.5EU(鱟試劑法)和10EU(顯色法)。LPS O55:B5可用于刺激人腹腔巨噬細胞(1ng/ml)以及馬腹腔巨噬細胞的活動(1-100ng/ml)。

外觀: 白色凍干粉
溶解性: 可溶于水(5mg/ml)或細胞培養(yǎng)基(1mg/ml),產(chǎn)生渾濁淡黃色溶液,溶于鹽溶液經(jīng)旋渦振蕩并加熱到70-80℃可得到一種濃度更高的LPS溶液(20mg/ml)。
1.  LPS粉末是非無菌的,用于細胞培養(yǎng)相關(guān)實驗需要過濾除菌,LPS儲存液的配置:將1mg  LPS重懸于1ml無菌平衡鹽溶液或細胞培養(yǎng)基,輕輕旋渦振蕩直至粉末完全溶解,即得到1mg/ml的儲存液。此儲存液可進一步用無菌平衡鹽或細胞培養(yǎng)基稀釋至需要的工作濃度。
2.  LPS儲存液的保存:本儲存液(1mg/ml)可放在4℃保存,約一個月穩(wěn)定;也可分裝成單次用量后放到-20℃,2年穩(wěn)定。避免反復凍融。
注意:LPS溶液應儲存于硅烷化容器內(nèi),因為LPS可吸附于塑料或某些玻璃器皿,尤其當其濃度<0.1mg/ml時。但當LPS濃度大于1mg/ml時,上述吸附則可忽略不計。2)如使用玻璃器皿,則需旋渦振蕩LPS溶液至少30min,以重溶被吸附溶質(zhì)。
儲存條件: 2-8℃
注意: 部分產(chǎn)品我司僅能提供部分信息,我司不保證所提供信息的權(quán)威性,僅供客戶參考交流研究之用。

參考文獻(135篇)

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